Cell Culture Media Ndi nsanja Yachitukuko Chokhazikika
Ma cell culture media ndi msuzi wopatsa thanzi womwe uli ndi michere yofunika komanso zinthu zomwe zimafunikira kuti ma cell akule komanso kusamalidwa.Nthawi zambiri amapangidwa ndi kusakaniza koyenera kwa chakudya, mapuloteni, lipids, mchere, mavitamini, ndi kukula.Mawayilesi amathandiziranso malo abwino kuti ma cell azitha kuyenda bwino, monga pH yabwino, kuthamanga kwa osmotic, ndi kutentha.Makanema amathanso kukhala ndi maantibayotiki oletsa kuipitsidwa ndi bakiteriya kapena mafangasi, ndi zina zowonjezera kuti zikule kukula kwa mitundu ina ya maselo.Makanema amtundu wama cell amagwiritsidwa ntchito pofufuza ndi zamankhwala osiyanasiyana, monga uinjiniya wa minofu, kupeza mankhwala, ndi kafukufuku wa khansa.
Tsinde Sell Culture Media
Stem cell culture media nthawi zambiri imakhala ndi kuphatikiza kwa basal medium, monga Dulbecco's Modified Eagle Medium (DMEM) kapena RPMI-1640, ndi seramu yowonjezera, monga fetal bovine serum (FBS).Sing'anga yoyambira imapereka zakudya zofunikira komanso mavitamini, pomwe seramu yowonjezera imawonjezera zinthu zakukula, monga insulin, transferrin ndi selenium.Kuphatikiza apo, ma stem cell media media amatha kukhala ndi maantibayotiki, monga penicillin, kuti apewe kuipitsidwa ndi mabakiteriya.Nthawi zina, zowonjezera zowonjezera, monga zowonjezera zowonjezera zowonjezera, zikhoza kuwonjezeredwa kuzinthu zofalitsa zachikhalidwe kuti ziwonjezere kukula kwa maselo a tsinde kapena kusiyanitsa.
Maselo a Embryonic Stem Cell
Maselo a Embryonic stem cell (ESCs) ndi ma cell a stem cell omwe amachokera mkati mwa cell mass ya blastocyst, kamwana kakang'ono koyambirira.Ma ESC a anthu amatchedwa ma hESC.Iwo ndi pluripotent, kutanthauza kuti amatha kusiyanitsa mu mitundu yonse ya maselo a magawo atatu a majeremusi: ectoderm, endoderm ndi mesoderm.Ndiwo chida chamtengo wapatali chophunzirira zamoyo zachitukuko, ndipo kugwiritsa ntchito kwawo mankhwala obwezeretsanso kuchiza matenda osiyanasiyana kwakhala cholinga cha kafukufuku wambiri.
