CHO Cell Line Perekani Ntchito Zosinthidwa Mwamakonda Anu
Mzere wa HEK293T (HEK293 wosinthidwa) ndi mzere wa cell wa embryonic wa impso wotengedwa kuchokera ku mluza wa munthu m'ma 1970.Amagwiritsidwa ntchito m'machitidwe osiyanasiyana a kafukufuku ndipo ndi chida chofunika kwambiri pofufuza za jini, kapangidwe ka mapuloteni ndi ntchito, kutulutsa zizindikiro, ndi kupeza mankhwala.Maselo ndi osavuta kupatsirana ndipo amagwiritsidwa ntchito pophunzira zotsatira za kusintha kwa majini kosiyanasiyana, monga kufotokoza mopambanitsa kapena kugwetsa ma jini osiyanasiyana, pa phenotype ya selo.Maselo agwiritsidwanso ntchito pofufuza za stem cell biology, biology ya khansa, ndi immunology.
Primary Cell Culture
Chikhalidwe choyambirira cha cell ndi njira yomwe imagwiritsidwa ntchito kukulitsa ndikusunga ma cell mu vitro kuchokera ku cell imodzi kapena gulu la maselo.Njirayi imagwiritsidwa ntchito pofufuza momwe maselo amakhalira komanso momwe thupi limakhalira, ndipo lingagwiritsidwe ntchito pazinthu zosiyanasiyana, kuphatikizapo kuyesa mankhwala, kufufuza zachipatala, ndi machiritso opangidwa ndi maselo.Chikhalidwe choyambirira cha cell imachitika m'malo olamulidwa, nthawi zambiri pa benchi ya labotale, ndipo imathandizidwa ndi zida zingapo zapadera ndi ma reagents.Maselo amasungidwa amoyo mwa kupereka zakudya zofunika ndi kusunga kutentha koyenera, pH, ndi mpweya wabwino.Maselo amayang'aniridwanso ngati pali zizindikiro zilizonse za kupsinjika maganizo kapena kuipitsidwa, ndipo chikhalidwe chimafufuzidwa nthawi zonse kuti chikhale chosinthika pakukula kapena morphology.
Selo la Munthu
Selo la munthu ndilo gawo lalikulu la moyo.Thupi la munthu limapangidwa ndi maselo mabiliyoni ambiri omwe ali ndi kapangidwe kake ndi ntchito yake.Maselo ndiye zitsulo zomangira zamoyo zonse ndipo amayang'anira kukula, metabolism, ndi njira zina zofunika.Maselo amapangidwa ndi zinthu zosiyanasiyana, kuphatikizapo mapuloteni, DNA, chakudya, lipids, ndi organelles.
Secondary Cell Culture
Secondary cell chikhalidwe ndi ndondomeko culturing maselo amene adzipatula ndipo anakulira mu labotale pamaso.Maselo amatha kupangidwa kuchokera kuzinthu zotulutsa minofu, kulekanitsidwa ndi ma enzyme, kapena kupangidwa kuchokera ku cell imodzi.Chikhalidwe cha ma cell achiwiri chimagwiritsidwa ntchito kukulitsa mizere ya ma cell, kuphunzira machitidwe a cell, ndikupanga zoyesa zotengera ma cell.Mitundu yodziwika bwino ya ma cell omwe amagwiritsidwa ntchito pama cell achiwiri amaphatikiza ma fibroblasts, ma endothelial cell, ndi maselo osalala a minofu.
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