Ma cell Line Apangidwa Osavuta Komanso Ogwira Ntchito

1.Kusankha mzere wolondola wa cell
Posankha mzere woyenerera wa cell kuti muyesere, chonde ganizirani izi:
Mitundu: Maselo omwe sianthu komanso omwe si anyani nthawi zambiri amakhala ndi zoletsa zochepa zachitetezo chachilengedwe, koma pamapeto pake kuyesa kwanu kudzatsimikizira ngati mungagwiritse ntchito chikhalidwe cha mtundu wina.
b.Zinthu: Cholinga cha kuyesa kwanu ndi chiyani?Mwachitsanzo, mizere yochokera ku chiwindi ndi impso ingakhale yoyenera kuyesa kawopsedwe.
c.Zochepa kapena mosalekeza: Ngakhale kusankha kuchokera pamzere wocheperako kungakupatseni zosankha zambiri zowonetsera ntchito yolondola, mizere yopitilira ya cell nthawi zambiri imakhala yosavuta kufananiza ndikuyisamalira.
d.Normal kapena kusinthidwa: Mizere yosinthidwa ya maselo nthawi zambiri imakhala ndi kukula kwakukulu komanso kumera bwino kwa mbeu, kumapitirizabe, ndipo kumafuna seramu yochepa mu chikhalidwe cha chikhalidwe, koma phenotype yawo yasintha kosatha kupyolera mu kusintha kwa majini.
e.Kukula ndi mawonekedwe: Kodi zomwe mukufuna pakukula mwachangu, kuchuluka kwa machulukitsidwe, kuchita bwino kwa cloning ndi kuthekera kwakukula koyimitsidwa ndi chiyani?Mwachitsanzo, kuti muwonetse mapuloteni ophatikizana ndi zokolola zambiri, mungafunike kusankha mizere ya maselo yomwe ili ndi ziŵerengero za kukula mofulumira komanso kuthekera kokulirapo mwa kuyimitsidwa.
f.Njira zina: Ngati mukugwiritsa ntchito ma cell ochepa, kodi pali katundu wokwanira?Kodi mzere wamaselo ndiwodziwika bwino, kapena muyenera kutsimikizira nokha?Ngati mukugwiritsa ntchito ma cell achilendo, kodi pali mzere wofanana wa cell womwe ungagwiritsidwe ntchito ngati chiwongolero?Kodi mzere wama cell ndi wokhazikika?Ngati sichoncho, ndizosavuta bwanji kuti mufanane ndi kupanga zinthu zozizira zokwanira zoyeserera zanu?

2.Pezani mizere yama cell
Mukhoza kupanga chikhalidwe chanu kuchokera ku maselo oyambirira, kapena mutha kusankha kugula zikhalidwe zokhazikika kuchokera kwa ogulitsa kapena osapindula (ie mabanki a cell).Ogulitsa olemekezeka amapereka mizere yamtundu wapamwamba kwambiri yomwe yayesedwa mosamala kuti ikhale yokhulupirika ndikuonetsetsa kuti chikhalidwecho chilibe zonyansa.Tikukulimbikitsani kuti tisabwereke zikhalidwe kuchokera ku ma laboratories ena chifukwa ali ndi chiopsezo chachikulu cha kuipitsidwa kwa chikhalidwe cha ma cell.Mosasamala kanthu komwe akuchokera, chonde onetsetsani kuti mizere yonse yatsopano ya cell yayesedwa kuti iipitsidwe ndi mycoplasma musanayambe kuigwiritsa ntchito.