1.Kodi chikhalidwe cha maselo ndi chiyani?
Chikhalidwe cha ma cell chimatanthawuza kuchotsa ma cell a nyama kapena zomera ndikuzikulitsa pamalo abwino opangira.Maselo amatha kutengedwa mwachindunji kuchokera ku minofu ndikuphwanyidwa ndi njira za enzymatic kapena makina asanayambe kulimidwa, kapena amachokera ku mizere yokhazikika ya maselo kapena ma cell.
2.Chikhalidwe choyambirira ndi chiyani?
Chikhalidwe choyambirira chimatanthawuza gawo la chikhalidwe pambuyo poti maselo asiyanitsidwa ndi minofu ndikuchulukana pansi pamikhalidwe yoyenera mpaka atatenga magawo onse omwe alipo (ndiko kuti, kufikira kuphatikizika).Panthawi imeneyi, ma cell amayenera kusinthidwa ndikusamutsidwa ku chidebe chatsopano chokhala ndi sing'anga yatsopano yokulirapo kuti apereke malo ochulukirapo opitilira kukula.
2.1 Mzere wa ma cell
Pambuyo pa subculture yoyamba, chikhalidwe choyambirira chimatchedwa cell line kapena subclone.Mizere ya ma cell yochokera ku miyambo yoyambirira imakhala ndi moyo wocheperako (ie ndi yochepa; onani pansipa), ndipo ikadutsa, maselo omwe ali ndi mphamvu yakukula kwambiri amalamulira, zomwe zimapangitsa kuti chiwerengero cha genotype chikhale chosagwirizana ndi phenotype.
2.2 Kuvuta kwa ma cell
Ngati kachulukidwe ka cell kasankhika bwino kuchokera pachikhalidwe popanga ma cloning kapena njira ina, mzere wa cell umakhala zovuta zama cell.Mitundu ya ma cell nthawi zambiri imakhala ndi kusintha kwina kwa chibadwa mzere wa makolo ukayamba.
3.Mizere yocheperako komanso yopitilira ma cell
Maselo achibadwa nthawi zambiri amagawaniza kangapo kochepa chabe asanathe kufalikira.Ichi ndi chochitika chodziwika bwino chotchedwa senescence;ma cell awa amatchedwa finite cell lines.Komabe, ma cell ena amakhala osakhoza kufa kudzera m’njira yotchedwa kusandulika, komwe kumangochitika zokha kapena kumabwera chifukwa cha mankhwala kapena mavairasi.Pamene mzere wotsirizira wa selo umasintha ndikupeza mphamvu yogawaniza mpaka kalekale, umakhala mzere wa selo wopitirira.
4.Chikhalidwe cha chikhalidwe
Zikhalidwe zamtundu uliwonse wa cell ndizosiyana kwambiri, koma malo opangira ma cell nthawi zonse amakhala ndi chidebe choyenera, chomwe chimakhala ndi izi:
4.1 Gawo laling'ono kapena chikhalidwe chomwe chimapereka zakudya zofunikira (ma amino acid, chakudya, mavitamini, mchere)
4.2 Zinthu zakukula
4.3 Mahomoni
4.4 Magesi (O2, CO2)
4.5 Kuwongolera chilengedwe ndi mankhwala (pH, kuthamanga kwa osmotic, kutentha)
Maselo ambiri amadalira nangula ndipo amayenera kulimidwa pagawo lolimba kapena lolimba (chikhalidwe chotsatira kapena chikhalidwe cha monolayer), pomwe maselo ena amatha kuyandama pakatikati (chikhalidwe choyimitsidwa).
5.Cryopreservation
Ngati pali maselo owonjezera mu subculture, ayenera kuthandizidwa ndi wothandizira oyenerera (monga DMSO kapena glycerol) ndi kusungidwa pa kutentha pansi -130 ° C (cryopreservation) mpaka akufunika.Kuti mudziwe zambiri za subculture ndi cryopreservation of cell.
6.Morphology ya maselo mu chikhalidwe
Maselo mu chikhalidwe atha kugawidwa m'magulu atatu ofunikira potengera mawonekedwe ndi mawonekedwe ake (ie morphology).
6.1 Maselo a Fibroblasts ndi ochititsa chidwi kapena ochulukirapo, amakhala ndi mawonekedwe otalikirapo, ndipo amakula molumikizana ndi gawo lapansi.
6.2 Maselo ngati epithelial ndi polygonal, amakhala ndi kukula kokhazikika, ndipo amamangiriridwa ku matrix m'mapepala a discrete.
6.3 Maselo ngati Lymphoblast ndi ozungulira ndipo nthawi zambiri amakula moyimitsidwa popanda kulumikiza pamwamba.
7.Kugwiritsa ntchito chikhalidwe cha ma cell
Chikhalidwe cha ma cell ndi chimodzi mwazida zazikulu zomwe zimagwiritsidwa ntchito mu cell and molecular biology.Amapereka njira yabwino kwambiri yophunzirira momwe thupi limakhalira komanso biochemistry ya maselo (monga kafukufuku wa kagayidwe kachakudya, ukalamba), zotsatira za mankhwala ndi mankhwala oopsa pama cell, ndi mutagenesis ndi carcinogenic zotsatira.Amagwiritsidwanso ntchito powunika mankhwala ndi chitukuko komanso kupanga kwakukulu kwazinthu zachilengedwe (monga katemera, mapuloteni achire).Ubwino waukulu wogwiritsa ntchito chikhalidwe cha maselo pazifukwa zilizonsezi ndi kusasinthika komanso kupangika kwa zotsatira zomwe zingapezeke pogwiritsa ntchito gulu la maselo opangidwa.
Nthawi yotumiza: Jun-03-2019