Kuphatikizika Kwapatsamba Molondola Lowetsani Ma Genes Amene Mukufuna Kumalo Enaake Otentha
Kuphatikizika kwa tsambali ndi njira yosinthira webusayiti kapena pulogalamu kuti igwirizane ndi zosowa zapadera za tsamba linalake kapena ntchito.Ndilo ndondomeko yomwe imaphatikizapo kusintha ma code omwe alipo komanso mapangidwe a webusaitiyi kapena ntchito kuti ikhale yogwira mtima komanso yogwira ntchito pa zosowa zenizeni za malo.Kuphatikizika kwa tsamba kutha kugwiritsidwa ntchito kusintha zomwe zilipo kale, kuwonjezera zatsopano, ndikusintha momwe tsambalo limagwirira ntchito kapena kugwiritsa ntchito.Izi ndizothandiza makamaka kwa mabizinesi omwe ali ndi mawebusayiti angapo kapena mapulogalamu omwe amayenera kuphatikizidwa ndi wina ndi mnzake kuti apatse makasitomala awo chidziwitso chosavuta.
Kuphatikizana kwapadera kwa malo mu maselo a CHO ndi njira yomwe imagwiritsidwa ntchito poyambitsa jini lachidwi kumalo odziwika bwino mu genome ya Chinese hamster ovary (CHO) maselo.Njirayi imaphatikizapo kugwiritsa ntchito enzyme yokhudzana ndi malo kuti igwirizane ndi ndondomeko yeniyeni ya CHO cell genome kenako ndikuphatikiza jini yachidwi muzotsatira zomwe mukufuna.Njirayi imalola kuwongolera bwino kwambiri pakuyika kwa majini mumtundu wa CHO cell genome ndipo ingathandize kupewa kuphatikizika mwachisawawa, komwe kumatha kukhala ndi zotsatira zoyipa pama cell.Ubwino waukulu wa njirayi ndikuti umapereka chiwongolero chachikulu komanso cholondola panjira yophatikizira, komanso kukhazikika kwakukulu kwa jini pakapita nthawi.Kuonjezera apo, njirayi ingagwiritsidwe ntchito poyambitsa majini angapo m'malo osiyanasiyana mkati mwa selo, ndikupangitsa kukhala chida champhamvu chogwiritsira ntchito majini.
Kulunjika ma vectors
Ma vector olunjika amagwiritsidwa ntchito popanga zamoyo zosinthidwa ma genetic poyambitsa ma DNA otsatizana mu ma genome awo.Nthawi zambiri amapangidwa ndi cholembera chibadwa chomwe chimalola kuzindikira maselo osinthidwa, chikhomo chomwe chimalola kusankha ma cell osinthidwa, ndi gawo lophatikizana lofanana lomwe limalola kuphatikizika kwa DNA yomwe mukufuna kutengera mtundu wa chamoyo chomwe mukufuna.Ma vector olunjika amagwiritsidwa ntchito kwambiri pochotsa ma jini, kugogoda kwa majini, kusintha ma gene, ndi mitundu ina ya uinjiniya wa majini.
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