Zipangizo Zamtundu Wamaselo Zimakweza Bwino Kukula Kwa Maselo

Zofunikira zenizeni za labotale ya chikhalidwe cha maselo makamaka zimadalira mtundu wa kafukufuku womwe ukuchitidwa;mwachitsanzo, zosowa za labotale yokhudzana ndi chikhalidwe cha ma mammalian cell yomwe imagwira ntchito pa kafukufuku wa khansa ndizosiyana kwambiri ndi za labotale yokhudzana ndi chikhalidwe cha tizilombo yomwe imayang'ana kwambiri za mapuloteni.Komabe, ma labotale onse a chikhalidwe cha ma cell ali ndi chofunikira chimodzi, ndiye kuti, palibe tizilombo toyambitsa matenda (ndiye kuti, wosabala), ndikugawana zida zina zofunika pa chikhalidwe cha ma cell.

Gawoli limatchula zida ndi zinthu zomwe zimagwiritsidwa ntchito m'ma laboratories ambiri amtundu wa ma cell, komanso zida zothandiza zomwe zingathandize kuti ntchitoyi ichitike bwino kapena molondola kapena kulola kuzindikirika ndi kusanthula kochulukira.

Chonde dziwani kuti mndandandawu siwokwanira;zofunikira za labotale iliyonse yama cell zimadalira mtundu wa ntchito yomwe yachitika.

1.Zida zoyambira
Chophimba cha cell culture (ie laminar flow hood kapena biological security cabinet)
Chofungatira (timalimbikitsa kugwiritsa ntchito chofungatira chonyowa cha CO2)
Kusamba madzi
Centrifuge
Mafiriji ndi mafiriji (-20°C)
Ma cell counter (mwachitsanzo, Countess automatic cell counter kapena blood cell counter)
Maikulosikopu otembenuzidwa
Mufiriji wa nayitrogeni wamadzi (N2) kapena chidebe chosungiramo kutentha pang'ono
Sterilizer (ie autoclave)

2.Zida zowonjezera ndi zowonjezera zowonjezera
Pampu yopumira (peristaltic kapena vacuum)
pH mita
Maikulosikopu ya Confocal
Kuthamanga kwa cytometer
Zotengera zama cell (monga ma flasks, mbale za petri, mabotolo odzigudubuza, mbale za zitsime zambiri)
Pipettes ndi pipettes
Syringe ndi singano
Chidebe cha zinyalala
Sing'anga, seramu ndi reagents
Maselo
Cell cube
EG bioreactor